RBT501 GE Syllabus
AKTU Biotechnology (BT) RBT-501GE Syllabus for all 5 units are provided below. Download link for BT 5th SEM RBT501 Genetic Engineering Syllabus is listed down for students to make perfect utilization and score maximum marks with our study materials.
UP Technical University Biotechnology (BT) 5th SEM RBT501 GE – Genetic Engineering Syllabus
RBT501: Genetic Engineering
Unit I
Gene cloning- concept and basic steps; Restriction modification enzymes used in recombinant DNA
technology, endonucleases, ligases and other enzymes useful in gene cloning; Vectors: plasmid,
bacteriophage and other viral vectors, cosmids, artificial chromosomes, yeast artificial chromosome,
Bacterial Artificial Chromosome, Ti plasmid, shuttle vectors, expression vectors; DNA delivery
methods; Construction of genomic and cDNA libraries; Techniques for selection, screening and
characterization of transformants (hybridization based techniques, expression & interaction based
techniques).
Unit II
Concept of PCR; DNA polymerases; primer designing, linkers, adapters, setting up PCR reactions;
Various types of PCR; Applications of PCR in disease diagnostics, forensic sciences and genetic
engineering.
Unit III
Gene expression in prokaryotes & eukaryotes, Tissue specific promoter, wound inducible promoters,
Strong and regulatable promoters, promoter analysis (EMSA and DNA footprinting), gene
expression profiling (real time PCR, SAGE, differential display, Microarray); DNA sequencing
methods; Molecular markers: RAPD, RFLP, AFLP, SNP; Site directed mutagenesis, gene silencing
techniques.
Unit IV
Applications of genetic engineering; Creation of recombinant microorganisms, transgenic plants and
animals; cloning of sheep (Dolly) & other mammals; applications in conservation; therapeutic vs.
reproductive cloning; ethical issues and the prospects for human cloning; Gene therapy; DNA drugs
and vaccines.
Unit V
Basic concepts of cell signaling, Extracellular signal molecule and their receptors, Operation of
Signaling molecules over various distances, Cellular response to specific combinations of
extracellular signal molecules; Nuclear receptor; Ion channel linked, G-protein mediated receptors,
Relay of signal by activated cell surface receptors via intracellular signaling proteins, Intracellular
Signaling proteins as molecular switches.
Text books and references
1. T.A Brown (2006). Gene cloning and DNA analysis, WILEY-BLACKWELL
2. Genetic Engineering by Dr Smita Rastogi & Dr Neelak Pathak, Oxford University Press
3. S.B Primrose (2001). Molecular biotechnology. Panima Publishing corporation, 2nd edition
4. Molecular Cloning, A laboratory Manual. Sambrook, J., Fritsch, E.F., Mariatis.3rd edition
5. Genetic Engineering, Priciples & Practice by Sandhya Mitra, McGraw Hill Education.
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Related Links
For AKTU RBT501 GE Previous Year Question Papers – Click here
For AKTU RBT501 GE Important Questions/Short Answers – Click here
For AKTU RBT501 GE Lecture Notes – Click here
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RBT501 Syllabus, GE Unit-wise Syllabus – BT 5th Semester
