RBT551 GEL Lap Syllabus
AKTU Biotechnology (BT) RBT551 GEL Syllabus for all experiments is provided below. Download link for BT 5th SEM RBT551 Genetic Engineering Lab Syllabus is listed down for students to make perfect utilization and score maximum marks with our study materials.
UP Technical University Biotechnology (BT) 5th SEM RBT551 GEL– Genetic Engineering Lab Syllabus
RBT551: Genetic Engineering Lab
1. Estimation of DNA by diphenylamine reaction
2. Determination of RNA by orcinol method
3. To isolate plant DNA using CTAB.
4. Elution of plant DNA fragment from agarose gel
5. To perform restriction digestion of λ DNA
6. Dephosphorylation of restriction enzyme digested vector pUC18.
7. To make bacterial cells competent for transformation
8. To perform of transformation of the desired bacterial strain with plasmid DNA
9. SDS-PAGE of proteins under reducing conditions (SDS-PAGE)
10. To perform Southern Hybridization
11. To perform ligation of λ EcoRI digest using T4 DNA ligase
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AKTU 5th SEM BT GEL Syllabus
RBT551 Genetic Engineering Lab Syllabus free download
UP Technical University BT GEL Syllabus
RBT551 Syllabus, GEL Unit-wise Syllabus – BT 5th Semester